WebPinPoint Xa-1 and pGEX-6P-1 expression vectors, inco rporating a biotin purification tag and a glutathione-S-transferase tag, respectively. These regions were then expressed in E. coli. Only the pGEX-6P-1 has been found suitable for serological studies as its tag showed less cross reactivity with human sera and was WebPinPoint Xa-1 T-Vector Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially …
Vector Database - Addgene
WebPinPointXa-1 Vector DB Sequence Link : General :3331 bp DNA Functions :protein expression Selection :ampR, beta-lactamase Copy Number : Hosts : Suppliers :Promega … Web# Primer Sequences (5' ~ 3') Plasmid Manufacturer 1 28 glll GTATGGGATTTTGCTAAACAAC Ph.D. glll New England Biolabs 1a -96 glll CCCTCATAGTTAGCGTAACG Ph.D. glll New England Biolabs 2 Ac5 Forward ACACAAAGCCGCTCCATCAG pAc5.1/V5-His Invitrogen 3 AD Reverse … scepter\u0027s hr
PinPoint Xa-3 - EcoliWiki
WebThe PinPoint Xa Protein Purification System is used for the production and purification of fusion proteins that are biotinylated in vivo. The PinPoint Vectors an encoded … WebPinPointTM Xa protein purification system Promega's newPinPointTM Xaprotein purification system is a simple, reliable non-denaturing methodto express and purify recombinant fusion proteins in E.coli. PinPoint Xa vectors contain a sequence whichbecomes biotinylated in E.coli. Thebiotinylated protein binds to SoftLinkTM resin whichis ... WebPinPoint™ Control Vector; lane 4, PinPoint™ Control Vector (+ rifampicin); lane 5, PinPoint™ Xa-3 Vector; lane 6, pGEM®-9Zf(–) Vector. 2. Product Components and Storage Conditions Product Cat.# E. coliT7 S30 Extract System for Circular DNA L1130 Each system contains sufficient reagents for 30 × 50µl reactions. Includes: rural flagstaff county