Oxonium ion glycan
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Oxonium ion glycan
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WebNational Center for Biotechnology Information WebHere, we have developed a new method for determining the glycan composition of N-linked glycopeptides fragmented by collisional or hybrid activation that uses multiple sources of information from the spectrum, including observed glycan B-type (oxonium) and Y-type ions and mass and precursor monoisotopic selection errors to discriminate between …
WebJan 1, 2016 · The oxonium ion-containing spectra were matched to the N-glycopeptide candidate database (comprising the identified N-glycan and glycosite-containing peptides) to assign all glycopeptide ... WebJul 31, 2024 · Briefly, the software processes the input protein or peptide sequences to generate ion libraries containing peptide b and y ions, glycopeptide Y ions, and/or oxonium ions of choice, and allows the user to select the retention times (RT) to be used for the peptide (s) and precursor masses. Key features of the software are described in detail …
WebNov 14, 2024 · Glycosylation represents a major post-translational modification of proteins involved in various biological processes, including transcription, differentiation, apoptosis, cell adhesion, receptor-ligand binding, as well as oncogenic transformation and immune response ( 1 – 4 ). WebStereochemical and positional glycan information can be extracted from glycopeptide MS/MS information 38,349 (TABle 4). For example, ratios of specific oxonium ions can be used to discriminate ...
Webto generate fragment ion spectra of the glycan residue oxonium ions. Accurate mass measurement of the glycan oxonium ions in selected glycopeptide MS/MS spectra was achieved by using a number of the neighboring peptide fragment ions as internal mass standards. Plausi-ble elemental formulas were then generated for each glycan greatly …
WebFeb 1, 2024 · The oxonium ions that are typically used to differentiate glycan isomers on other types of mass spectrometers fall outside the lower mass range of our instrumental … townshend\u0027s tea flightsWebJul 5, 2024 · In this work, we first optimize the fragmentation settings with two purified glycoproteins for high quality fragmentation spectra boasting highly visible diagnostic glycan fragments, i.e., the glycan oxonium ions, as well as highly informative peptide backbone cleavages that can be used to confidently identify both the peptides and the … townshends solicitors coleshillWebCardiology. General Cardiology. 0.15 miles from Issaquah, WA. 3100 Northup Way, Bellevue, WA, 98004. Hospitals: UW Medicine-Harborview Medical Center + 1 townshends tea bozemanWebglycan oxonium ions at m/z’s such as 138.055, 204.087, and/ or 274.092. As shown previously by Medzihradszky et al. (21), ordinary and especially phospho-proteomics data sets often contain unrecognized glycopeptide spectra that can be found by filtering for oxonium ions, and it is quite feasible to enrich townshends tea flightsWebFocusing on the lower mass range that covers the oxonium ions for (B) O-and (E) N-glycans shows that these two glycosylation types create very different patterns of fragment ions … townshends businessWebThe difference in the oxonium ion fragmentation profiles of such glycopeptides may thus be used to distinguish among these glycan structures and could be of importance in LC–MS/MS-based glycoproteomic studies. KEYWORDS: glycoproteomics oxonium ion LC−MS/MS glycopeptide O-glycosylation Supporting Information townshineWebOxonium ions are generated after data-independent, all ion fragmentation (AIF) of glycopeptides during LC-MS/MS analysis, and are extracted from the MS/MS data to … townshill