Web05. jun 2006. · The terminator sequences for the antisense pg gene were the tml gene (T-tml) and the transcript 7 gene from the octopine-type Ti plasmid pTiA6 from A. tumefaciens. Expression of the nptII gene was under the control of the 5' promoter and 3' terminator sequences from the mannopine synthase gene derived from A. tumefaciens or the 35s … Web克隆菌株：大肠杆菌DB3.1. 质粒简介： pEarleyGate 301 (C-HA)是一个植物细胞Gateway系统表达质粒。. pEarleyGate 301 allows for rapid recombinational cloning of DNA from …

The 35S promoter used in a selectable marker gene of a plant

Web01. jul 2024. · Binary vector pC1300-Cas9 contains the 2 × 35S or MAS promotor, a codon-optimized Cas9 coding sequence, and the CaMV terminator. (B) Vector construction … Web14. avg 2015. · The increasing number and diversity of genetically modified organisms (GMOs) developed and commercialised forces laboratories to apply many different methods of analysis. Matrix-based approach helps to minimize analytical effort reducing the number of identifications. However, the correctness of screening phase needs efficient methods. … bauhaus umeå

Nopaline - an overview ScienceDirect Topics

WebA 479-bp bi-directional promoter controls the expression of two genes (mas1' and mas2') that encode enzymes for the synthesis of the opine mannopine in plant tissues infected … WebA Cas9/sgRNA expression cassette contains a 35S promoter driving the expression of Cas9 translationally fused to eGFP, followed by nopaline synthase terminator (Tnos); as well as Arabidopsis U6 ... Websynthase terminator (ocsT) to yield pPZP200/RB-p35S-BAR-ocsT-LB, and performed a similar analysis. In this case, 10 out of 172 T1 plants exhibited the long-hypocotyl phenotype (silencing rate5.8%) (Figure 2). These results indicate that replacing either the promoter or the terminator sequence alone could dramatically reduce TGS. tim gant obit